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gene knockout mutants of fimh  (Pfizer Inc)

 
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    Structured Review

    Pfizer Inc gene knockout mutants of fimh
    ( A ) Intracellular bacteria quantified by gentamicin protection assay 12 hpi with nonpathogenic E. coli (COM1, COM3, and HM50), UPEC (ClinA, <t>EC10,</t> <t>CFT073,</t> and respective ∆ <t>fimH</t> mutants), and KP at an MOI of 100 [limit of detection (LOD) = 0 CFU/ml]. Data plotted as mean of four independent biological replicates ( N = 4), ** P < 0.01; * P < 0.1. ( B ) Invasion without IBC formation by ∆ fimH in EC10 and CFT073 backgrounds (arrowheads). Scissors depict the position of cross sections for the side views for ∆ fimH EC10 in the adjacent panels. Staining as in <xref ref-type=Fig. 1 . Confocal images representative of a minimum of four independent biological replicates per strain ( N ≥ 4). " width="250" height="auto" />
    Gene Knockout Mutants Of Fimh, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+knockout+mutants+fimh/gene+knockout+mutants+of+fimh/pmc10631729-171-4-8
    Average 90 stars, based on 1 article reviews
    gene knockout mutants of fimh - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "A human urothelial microtissue model reveals shared colonization and survival strategies between uropathogens and commensals"

    Article Title: A human urothelial microtissue model reveals shared colonization and survival strategies between uropathogens and commensals

    Journal: Science Advances

    doi: 10.1126/sciadv.adi9834

    ( A ) Intracellular bacteria quantified by gentamicin protection assay 12 hpi with nonpathogenic E. coli (COM1, COM3, and HM50), UPEC (ClinA, EC10, CFT073, and respective ∆ fimH mutants), and KP at an MOI of 100 [limit of detection (LOD) = 0 CFU/ml]. Data plotted as mean of four independent biological replicates ( N = 4), ** P < 0.01; * P < 0.1. ( B ) Invasion without IBC formation by ∆ fimH in EC10 and CFT073 backgrounds (arrowheads). Scissors depict the position of cross sections for the side views for ∆ fimH EC10 in the adjacent panels. Staining as in <xref ref-type=Fig. 1 . Confocal images representative of a minimum of four independent biological replicates per strain ( N ≥ 4). " title="... HM50), UPEC (ClinA, EC10, CFT073, and respective ∆ fimH mutants), and KP at an MOI of 100 ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: ( A ) Intracellular bacteria quantified by gentamicin protection assay 12 hpi with nonpathogenic E. coli (COM1, COM3, and HM50), UPEC (ClinA, EC10, CFT073, and respective ∆ fimH mutants), and KP at an MOI of 100 [limit of detection (LOD) = 0 CFU/ml]. Data plotted as mean of four independent biological replicates ( N = 4), ** P < 0.01; * P < 0.1. ( B ) Invasion without IBC formation by ∆ fimH in EC10 and CFT073 backgrounds (arrowheads). Scissors depict the position of cross sections for the side views for ∆ fimH EC10 in the adjacent panels. Staining as in Fig. 1 . Confocal images representative of a minimum of four independent biological replicates per strain ( N ≥ 4).

    Techniques Used: Bacteria, Staining

    Related Articles

    Gene Knockout:

    Article Title: A human urothelial microtissue model reveals shared colonization and survival strategies between uropathogens and commensals
    Article Snippet: The MLST ST131 cystitis isolates EC6 and EC10 ( ) were obtained from Pfizer (Department of Vaccine Design, Immunology, and Anti-Infectives Pearl River, NY, USA). .. Gene knockout mutants of fimH were generated at Pfizer from CFT073 and EC10 parental strains using the λ-red–mediated homologous recombination system as previously described ( ). ..

    Generated:

    Article Title: A human urothelial microtissue model reveals shared colonization and survival strategies between uropathogens and commensals
    Article Snippet: The MLST ST131 cystitis isolates EC6 and EC10 ( ) were obtained from Pfizer (Department of Vaccine Design, Immunology, and Anti-Infectives Pearl River, NY, USA). .. Gene knockout mutants of fimH were generated at Pfizer from CFT073 and EC10 parental strains using the λ-red–mediated homologous recombination system as previously described ( ). ..

    Homologous Recombination:

    Article Title: A human urothelial microtissue model reveals shared colonization and survival strategies between uropathogens and commensals
    Article Snippet: The MLST ST131 cystitis isolates EC6 and EC10 ( ) were obtained from Pfizer (Department of Vaccine Design, Immunology, and Anti-Infectives Pearl River, NY, USA). .. Gene knockout mutants of fimH were generated at Pfizer from CFT073 and EC10 parental strains using the λ-red–mediated homologous recombination system as previously described ( ). ..



    Similar Products

    gene knockout mutants of fimh  (Pfizer Inc)
    90
    Pfizer Inc gene knockout mutants of fimh
    ( A ) Intracellular bacteria quantified by gentamicin protection assay 12 hpi with nonpathogenic E. coli (COM1, COM3, and HM50), UPEC (ClinA, <t>EC10,</t> <t>CFT073,</t> and respective ∆ <t>fimH</t> mutants), and KP at an MOI of 100 [limit of detection (LOD) = 0 CFU/ml]. Data plotted as mean of four independent biological replicates ( N = 4), ** P < 0.01; * P < 0.1. ( B ) Invasion without IBC formation by ∆ fimH in EC10 and CFT073 backgrounds (arrowheads). Scissors depict the position of cross sections for the side views for ∆ fimH EC10 in the adjacent panels. Staining as in <xref ref-type=Fig. 1 . Confocal images representative of a minimum of four independent biological replicates per strain ( N ≥ 4). " width="250" height="auto" />
    Gene Knockout Mutants Of Fimh, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+knockout+mutants+fimh/gene+knockout+mutants+of+fimh/pmc10631729-171-4-8
    Average 90 stars, based on 1 article reviews
    gene knockout mutants of fimh - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    gene knockout mutants fimh  (Pfizer Inc)
    90
    Pfizer Inc gene knockout mutants fimh
    ( A ) Intracellular bacteria quantified by gentamicin protection assay 12 hpi with nonpathogenic E. coli (COM1, COM3, and HM50), UPEC (ClinA, <t>EC10,</t> <t>CFT073,</t> and respective ∆ <t>fimH</t> mutants), and KP at an MOI of 100 [limit of detection (LOD) = 0 CFU/ml]. Data plotted as mean of four independent biological replicates ( N = 4), ** P < 0.01; * P < 0.1. ( B ) Invasion without IBC formation by ∆ fimH in EC10 and CFT073 backgrounds (arrowheads). Scissors depict the position of cross sections for the side views for ∆ fimH EC10 in the adjacent panels. Staining as in <xref ref-type=Fig. 1 . Confocal images representative of a minimum of four independent biological replicates per strain ( N ≥ 4). " width="250" height="auto" />
    Gene Knockout Mutants Fimh, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+knockout+mutants+fimh/gene+knockout+mutants+of+fimh/pmc10631729-171-0-8
    Average 90 stars, based on 1 article reviews
    gene knockout mutants fimh - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Intracellular bacteria quantified by gentamicin protection assay 12 hpi with nonpathogenic E. coli (COM1, COM3, and HM50), UPEC (ClinA, EC10, CFT073, and respective ∆ fimH mutants), and KP at an MOI of 100 [limit of detection (LOD) = 0 CFU/ml]. Data plotted as mean of four independent biological replicates ( N = 4), ** P < 0.01; * P < 0.1. ( B ) Invasion without IBC formation by ∆ fimH in EC10 and CFT073 backgrounds (arrowheads). Scissors depict the position of cross sections for the side views for ∆ fimH EC10 in the adjacent panels. Staining as in <xref ref-type=Fig. 1 . Confocal images representative of a minimum of four independent biological replicates per strain ( N ≥ 4). " width="100%" height="100%">

    Journal: Science Advances

    Article Title: A human urothelial microtissue model reveals shared colonization and survival strategies between uropathogens and commensals

    doi: 10.1126/sciadv.adi9834

    Figure Lengend Snippet: ( A ) Intracellular bacteria quantified by gentamicin protection assay 12 hpi with nonpathogenic E. coli (COM1, COM3, and HM50), UPEC (ClinA, EC10, CFT073, and respective ∆ fimH mutants), and KP at an MOI of 100 [limit of detection (LOD) = 0 CFU/ml]. Data plotted as mean of four independent biological replicates ( N = 4), ** P < 0.01; * P < 0.1. ( B ) Invasion without IBC formation by ∆ fimH in EC10 and CFT073 backgrounds (arrowheads). Scissors depict the position of cross sections for the side views for ∆ fimH EC10 in the adjacent panels. Staining as in Fig. 1 . Confocal images representative of a minimum of four independent biological replicates per strain ( N ≥ 4).

    Article Snippet: Gene knockout mutants of fimH were generated at Pfizer from CFT073 and EC10 parental strains using the λ-red–mediated homologous recombination system as previously described ( ).

    Techniques: Bacteria, Staining